Zeiss AxioImager. M2 upright fluorescence microscope with Apo Tome option

Zeiss AxioImager

- Fluorescence filter cubes installed

Filter cube Excitation range Excitation filter Emission filter Examples of dyes imaged Zeiss Part #
Filter Set 49/DAPI UV 365 BP 445/50 Alexa 350, DAPI, Hoechst, LysoTracker Blue 4880499901000000
Filter Set 15 green BP 546/12 LP 590 Fluor-Ruby, rhodamine, propidium iodide 48801150000000000
Filter Set 38/ GFP blue BP 470/40 BP 525/50 Alexa488, DiO, eCFR, eGFP, FITC, FM1-43, MitoTracker Green 4890389901000000

- Objective lenses installed

Magnification Type N.A. Immersion Working distance Zeiss part #
10Χ EC Plan-Neofluar 0.30 Air 5.2 μm 4203409901000000
20X Plan Apochromat 0.8 Air N/A 4206509900000000
40X EC Plan-Neofluar 0.75 Air 0.71 μm 4203609900000000
63X Plan Apochromat 1.40 Oil N/A 4207809900000000

- DIC Nomarski optics available.
- Epifluorescence excitation source: X-Cite metal halide bulb
- ApoTome option available.
- AxioVision 4 image acquisition software available with z-stack, multichannel, and ApoTome modules.

The microscope is equipped with a 1.4 megapixel Zeiss AxioCam MRm monochrome CCD camera with 1388 (H) x 1040 (V) pixels and 6.45 µm x 6.45 µm pixel size.

Location: D331C