Title: Who done it? A criminal investigation using indicator tests.
Topic: Indictor testing for the presence of protein, carbohydrates, and lipids.
Grade Level: Living Environment
Time: 80 minute double period.
First period- lecture
Second period- lab
Science background material for the teacher: All living things contain organic macromolecules: lipids, proteins, carbohydrates, and nucleic acids. Each of these macromolecules contain building blocks:
Testing for macromolecules:
Indicator: a substance that undergoes a distinct observable change when condition in its solution change. It indicates the presence or absence of a particular substance.
Objectives: At the end of this lesson students will be able to:
National Science Education Standards:
New York State Living Environment Core Curriculum Standards:
2.2a Development of a research plan involves researching background information and understanding the major concepts in the area being investigated. Recommendations for methodologies, use of technologies, proper equipment, and safety precautions should also be included.
1.2hMany organic and inorganic substances dissolved in cells allow necessary chemical reactions to take place in order to maintain life. Large organic food molecules such as proteins and starches must initially be broken down (digested to amino acids and simple sugars respectively), in order to enter cells. Once nutrients enter a cell, the cell will use them as building blocks in the synthesis of compounds necessary for life.
40 1mL pipettes
40 medium test tubes
Lugol’s iodine solution
1-package brown paper towels
500mL yogurt/water solution
500 mL milk/water solution
500 mL blended meat/water solution
500 mL corn starch/water solution
500 mL bread/water solution
Water (to act as control)
Computer (for teacher)
Projector/Smart Board (for teacher)
Who Done it? Video
Indicator solution Video
Motivation: Bring in various food products. Have the students identify what macromolecule the food is made of. Apple- carbohydrates. Milk- protein. Yogurt- protein. Bread- carbohydrates. Oil- lipids
Do Now: Think, Pair, Share: What do you think an indicator is? Indicator: a substance that undergoes a distinct observable change when condition in its solution change. It indicates the presence or absence of a particular substance.
Adaptations for students with disabilities: The lab area will be easily accessible for students with physical disabilities. This includes setting up the stations in a manner that the students can easily and safely rotate around. The use of a murder mystery in the beginning of the lesson will keep the attention of students with a general learning disability. Laptops and headphones will be made available for students that need to watch the videos at their own pace or re-watch the video to understand the material.
Multicultural Connections: Throughout out our discussion on macromolecules and different food groups the teacher can encourage students to share foods from back home that we may not have here and its food group.
Technology Incorporation: The teacher will use the projector and a saved movie to motivate the students. The teacher can also use the Smart Document Camera to display student work when going over the worksheet.
Assessment: Students will be assessed based on the completion of their work in the lab and the worksheet they hand in will be graded. They will also be given homework to complete on building blocks, macromolecules, and indicator testing.Extension Activities: Students can be given a research activity on the different indicator tests (such as cabbage juice as an indicator for pH).